The Landick Lab
University of Wisconsin-Madison
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Charles Yanofsky Professor
of Biochemistry & Bacteriology
5441 Microbial Sciences
1550 Linden Dr.
University of Wisconsin
Madison, WI 53706-1567
Ph. 608 265 8475
Fax 608 262 9865
University of Wisconsin-Madison
Department of Biochemistry
Department of Bacteriology
Department of Biomolecular Chemistry
iPIB - Integrated Program in Biochemistry
Microbiology Doctoral Training Program
CMB Training Program
Microbial Genome Biology Focus Group (CMB)
Genetics Training Program
Biophysics Training Program
Molecular Biosciences Training Program
Biotechnology Training Program
Great Lakes Bioenergy Research Center
Micro/Biochem 612 Resources
Welcome to the Landick Lab
Our research focuses on RNA polymerase, the central enzyme of gene expression in all free-living organisms. Our goal is to understand how RNA polymerase is regulated during the process of transcription (RNA synthesis). In organisms from bacteria to humans, the cell's ability to make long RNA chains, which include most mRNAs and some structural RNAs (e.g., rRNA), requires that extrinsic elongation regulators interact with RNA polymerase to suppress its innate tendency to fall into inactive off-line states that include long pauses, arrest, or termination. We seek to understand the fundamental properties of RNA polymerase that make it susceptible to pausing, arrest, or termination and how elongation regulators alter these properties. We study RNA polymerases from both bacterial and human cells and use a variety of approaches, from genetics to biophysics to structural biology, to study this fundamental paradigm of gene regulation. Lab members are engaged in experiments ranging from detailed biochemical characterization of protein-nucleic acid interactions, to the study of transcription regulators in cells using genome-scale methods (ChIPseq & RNAseq), to collaborative projects with other labs to study transcription by single molecules of RNA polymerase and to obtain crystallographic sturctures of RNA polymerase and transcription regulators. Our work has practical applications in drug discovery by identification on novel RNA polymerase inhibitors and in controlling transcriptional programs for synthetic microbiology. Follow links here to learn more about our research and our lab.
Check out our latest publications
Burmann BM, Knauer SH, Sevostyanova A, Schweimer K, Mooney RA, Landick R, Artsimovitch I, Ršsch P. 2012. An &alpha-helix to &beta-barrel domain switch transforms the transcription factor RfaH into a translation factor. Cell 150, 291-303.
Larson MH, Zhou J, Kaplan CD, Palangat M, Kornberg RD, Landick R, Block SM.. 2012. Trigger loop dynamics mediate the balance between the transcriptional fidelity and speed of RNA polymerase II. Proc. Natl. Acad. Sci. USA 109, 6555-7560.
Palangat,, M., J.A. Grass, M.F. Langelier, B. Coulombe and R. Landick. 2011. The RPB2 Flap Loop of Human RNA Polymerase II Is Dispensable for Transcription Initiation and Elongation Mol. Cell Biol. 31, 3312.
Conrad T.M., M. Frazier, A.R. Joyce, B.K. Cho, E.M. Knight, N.E. Lewis, R. Landick and B.O. Palsson. 2010. RNA polymerase mutants found through adaptive evolution reprogram Escherichia coli for optimal growth in minimal media. Proc. Natl. Acad. Sci. U.S.A. 107, 20500-20505.
Opalka, N., J. Brown, W.J. Lane, K.A. Twist, R. Landick, F.J. Asturias, S.A. Darst. 2010. Complete structural model of Escherichia coli RNA polymerase from a hybrid approach. PLoS Biol. 8, pii:e1000483
Ha, K.S., I. Toulokhonov, D.G. Vassylyev, and R. Landick. 2010. The NusA N-terminal domain is necessary and sufficient for enhancement of transcriptional pausing via interaction with the RNA exit channel of RNA polymerase. J. Mol. Biol. 401, 708-725.
Herbert K.M., Zhou J., Mooney R.A., Porta A.L., Landick R., and S.M. Block. 2010. E. coli NusG inhibits backtracking and accelerates pause-free transcription by promoting forward translocation of RNA polymerase.. J. Mol. Biol. 399, 17-30.