The Landick Lab
University of Wisconsin-Madison
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Charles Yanofsky Professor
of Biochemistry & Bacteriology
5441 Microbial Sciences
1550 Linden Dr.
University of Wisconsin
Madison, WI 53706-1567
Ph. 608 265 8475
Fax 608 262 9865
University of Wisconsin-Madison
Department of Biochemistry
Department of Bacteriology
Department of Biomolecular Chemistry
IPiB - Integrated Program in Biochemistry
Microbiology Doctoral Training Program
CMB Training Program
Genetics Training Program
Microbial Genome Biology Focus Group (CMB)
Biophysics Training Program
Molecular Biosciences Training Program
Biotechnology Training Program
Great Lakes Bioenergy Research Center
Micro/Biochem 612 Resources
Landick Lab News
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Welcome to the Landick Lab
Our research focuses on (1) RNA polymerase, the central enzyme of gene expression in all free-living organisms; (2) mechanisms by which gene expression by RNA polymerase is regulated and can be re-programmed for biodesign; and (3) applications of these basic research advances to microbial biotechnology and to antibiotic discovery. Our basic research focus is to understand how the fundamental properties of RNA polymerase, largely conserved from bacteria to human, make it susceptible to pausing, arrest, or termination and how elongation regulators, nucleoprotein structures, and metabolic, developmental, and environmental signals alter these properties. We use a variety of approaches, including genetics, biomolecular chemistry, synthetic biology, systems biology, biophysics, and structural biology, to study both fundamental and applied paradigms of gene regulation. Lab members develop and apply expertise on one or more approach to both individual and collaborative projects. Follow links here to learn more about our research and our lab.
Check out our latest publications
Mishanina TV, Palo MZ, Nayak D, Mooney RA, Landick R. 2017. Trigger loop of RNA polymerase is a positional, not acid-base, catalyst for both transcription and proofreading. Proc. Natl. Acad. Sci. U. S. A., 114, E5103-E5112. (incl. supplement)
Feklistov A, Bae B, Hauver J, Lass-Napiorkowska A, Kalesse M, Glaus F, Altmann KH, Heyduk T, Landick R, Darst SA. 2017. RNA polymerase motions during promoter melting. Science., 356, 863-866. (incl. supplement; Movie S1)
Steinert H, Sochor F, Wacker A, Buck J, Helmling C, Hiller F, Keyhani S, Noeske J, Grimm S, Rudolph MM, Keller H, Mooney RA, Landick R, Suess B, Furtig B, Wohnert J, Schwalbe H. 2017. Pausing guides RNA folding to populate transiently stable RNA structures for riboswitch-based transcription regulation. Elife., 6, e21297. (incl. supplement)
Kohler R, Mooney RA, Mills DJ, Landick R, Cramer P. 2017. Architecture of a transcribing-translating expressome. Science., 356, 194-197. (incl. supplement)
Tetone LE, Friedman LJ, Osborne ML, Ravi H, Kyzer S, Stumper SK, Mooney RA, Landick R, Gelles J. 2017. Dynamics of GreB-RNA polymerase interaction allow a proofreading accessory protein to patrol for transcription complexes needing rescue. Proc. Natl. Acad. Sci. U. S. A., 114, E1081-E1090. (incl. supplement)
Sato TK, Tremaine M, Parreiras LS, Hebert AS, Myers KS, Higbee AJ, Sardi M, McIlwain SJ, Ong IM, Breuer RJ, Narasimhan RA, McGee MA, Dickinson Q, La Reau A, Xie D, Tian M, Piotrowski JS, Reed JL, Zhang Y, Coon JJ, Hittinger CT, Gasch AP, Landick R. 2016. Directed evolution reveals unexpected epistatic interactions that alter metabolic regulation and enable anaerobic xylose use by Saccharomyces cerevisiae. PLoS Genet., 12, e1006372. (incl. supplement and erratum, published in PLoS Genet., 12, e1006447.)
Ghosh IN, Landick R. 2016. OptSSeq: High-throughput sequencing readout of growth enrichment defines optimal gene expression elements for homoethanologenesis. ACS Synth. Biol., 5, 1519-1534. (incl. supplement)
McIlwain SJ, Peris D, Sardi M, Moskvin OV, Zhan F, Myers K, Riley NM, Buzzell A, Parreiras LS, Ong IM, Landick R, Coon JJ, Gasch AP, Sato TK, Hittinger CT. 2016. Genome sequence and analysis of a stress-tolerant, wild-derived strain of Saccharomyces cerevisiae used in biofuels research. G3 (Bethesda)., 6, 1757-1766. (incl. supplement; supplemental tables)
Ray-Soni A, Bellecourt MJ, Landick R. 2016. Mechanisms of bacterial transcription termination: all good things must end. Annu. Rev. Biochem., 85, 319-347.
Zhang J, Landick R. 2016. A two-way street: regulatory interplay between RNA polymerase and nascent RNA structure. Trends Biochem. Sci. 41, 293-310.
Ronayne EA, Wan YC, Boudreau BA, Landick R, Cox MM. 2016. P1 ref endonuclease: a molecular mechanism for phage-enhanced antibiotic lethality. PLoS Genet. 12, e1005797.
Harden TT, Wells CD, Friedman LJ, Landick R, Hochschild A, Kondev J, Gelles J. 2016. Bacterial RNA polymerase can retain sigma70 throughout transcription. Proc. Natl. Acad. Sci. U. S. A. 113, 602-607. (incl. supplement)
Bae B, Feklistov A, Lass-Napiorkowska A, Landick R, Darst SA. 2015. Structure of a bacterial RNA polymerase holoenzyme open promoter complex. eLife 4, e08504. (incl. supplement)
Bae B, Nayak D, Ray A, Mustaev A, Landick R, Darst SA. 2015. CBR antimicrobials inhibit RNA polymerase via at least two bridge-helix cap-mediated effects on nucleotide addition. Proc. Natl. Acad. Sci. U. S. A. 112, E4178-E4187. (incl. supplement)
Kotlajich MV, Hron DR, Boudreau BA, Sun Z, Lyubchenko YL, Landick R. 2015. Bridged filaments of histone-like nucleoid structuring protein pause RNA polymerase and aid termination in bacteria. eLife. 4, e04970.
Windgassen TA, Mooney RA, Nayak D, Palangat M, Zhang J, Landick R. 2014. Trigger-helix folding pathway and SI3 mediate catalysis and hairpin-stabilized pausing by Escherichia coli RNA polymerase. Nucleic Acids Res. 42, 12707-12721. (incl. supplement)
Hein PP, Kolb KE, Windgassen T, Bellecourt MJ, Darst SA, Mooney RA, Landick R. 2014. RNA polymerase pausing and nascent-RNA structure formation are linked through clamp-domain movement. Nat. Struct. Mol. Biol. 21, 794-802. (incl. supplement)
Haft RJ, Keating DH, Schwaegler T, Schwalbach MS, Vinokur J, Tremaine M, Peters JM, Kotlajich MV, Pohlmann EL, Ong IM, Grass JA, Kiley PJ, Landick R. 2014. Correcting direct effects of ethanol on translation and transcription machinery confers ethanol tolerance in bacteria. Proc. Natl. Acad. Sci. U. S. A. 111, E2576-E2585. (incl. supplement)
Larson MH, Mooney RA, Peters JM, Windgassen T, Nayak D, Gross CA, Block SM, Greenleaf WJ, Landick R, Weissman JS. 2014. A pause sequence enriched at translation start sites drives transcription dynamics in vivo. Science. 344, 1042-1047. (incl. supplement)
Czyz A, Mooney RA, Iaconi A, Landick R. 2014. Mycobacterial RNA polymerase requires a U-tract at intrinsic terminators and is aided by NusG at suboptimal terminators. MBio. 5, e00931. (incl. supplement)
Zhang Y, Mooney RA, Grass JA, Sivaramakrishnan P, Herman C, Landick R, Wang JD. 2014. DksA guards elongating RNA polymerase against ribosome-stalling-induced arrest. Mol. Cell. 53, 766-778. (incl. supplement)
Kolb KE, Hein PP, Landick R. 2014. Antisense oligonucleotide-stimulated transcriptional pausing reveals RNA exit channel specificity of RNA polymerase and mechanistic contributions of NusA and RfaH. J. Biol. Chem. 289, 1151-1163.
Full publications list