The Landick Lab
University of Wisconsin-Madison
 
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Robert Landick
Charles Yanofsky Professor
of Biochemistry & Bacteriology
5441 Microbial Sciences
1550 Linden Dr.
University of Wisconsin
Madison, WI 53706-1567

Ph. 608 265 8475
Fax 608 262 9865



Program Links
University of Wisconsin-Madison
Department of Biochemistry
Department of Bacteriology
Department of Biomolecular Chemistry
IPiB - Integrated Program in Biochemistry
Microbiology Doctoral Training Program
CMB Training Program
Genetics Training Program
Microbial Genome Biology Focus Group (CMB)
Biophysics Training Program
Molecular Biosciences Training Program
Biotechnology Training Program
Biotechnology Center
Great Lakes Bioenergy Research Center
Micro/Biochem 612 Resources


Landick Lab News


Welcome to the Landick Lab

Our research focuses on (1) RNA polymerase, the central enzyme of gene expression in all free-living organisms; (2) mechanisms by which gene expression by RNA polymerase is regulated and can be re-programmed for biodesign; and (3) applications of these basic research advances to microbial biotechnology and to antibiotic discovery. Our basic research focus is to understand how the fundamental properties of RNA polymerase, largely conserved from bacteria to human, make it susceptible to pausing, arrest, or termination and how elongation regulators, nucleoprotein structures, and metabolic, developmental, and environmental signals alter these properties. We use a variety of approaches, including genetics, biomolecular chemistry, synthetic biology, systems biology, biophysics, and structural biology, to study both fundamental and applied paradigms of gene regulation. Lab members develop and apply expertise on one or more approach to both individual and collaborative projects. Follow links here to learn more about our research and our lab.

Check out our latest publications

Kang JY, Mooney RA, Nedialkov Y, Saba J, Mishanina TV, Artsimovitch I, Landick R, Darst SA. 2018. Structural basis for transcript elongation control by NusG family universal regulators. Cell., 173, 1650-1662.e14. (incl. supplement)

Yang S, Vera JM, Grass J, Savvakis G, Moskvin OV, Yang Y, McIlwain SJ, Lyu Y, Zinonos I, Hebert AS, Coon JJ, Bates DM, Sato TK, Brown SD, Himmel ME, Zhang M, Landick R, Pappas KM, Zhang Y. 2018. Complete genome sequence and the expression pattern of plasmids of the model ethanologen Zymomonas mobilis ZM4 and its xylose-utilizing derivatives 8b and 2032. Biotechnol. Biofuels., 11, 125.

Boudreau BA, Hron DR, Qin L, van der Valk RA, Kotlajich MV, Dame RT, Landick R. 2018. StpA and Hha stimulate pausing by RNA polymerase by promoting DNA-DNA bridging of H-NS filaments. Nucleic Acids Res., 46, 5525-5546. (incl. supplement)

Helmling C, Klotzner DP, Sochor F, Mooney RA, Wacker A, Landick R, Furtig B, Heckel A, Schwalbe H. 2018. Life times of metastable states guide regulatory signaling in transcriptional riboswitches. Nat. Commun., 9, 944. (incl. supplement)

Kang J, Mishanina TV, Bellecourt MJ, Mooney RA, Darst SA, Landick R. 2018. RNA polymerase accommodates a pause RNA hairpin by global conformational rearrangements that prolong pausing. Mol. Cell., 69, 802-815.e1. (incl. supplement; Movie S1; Movie S2)

Boyaci H, Chen J, Lilic M, Palka M, Mooney RA, Landick R, Darst SA, Campbell EA. 2018. Fidaxomicin jams Mycobacterium tuberculosis RNA polymerase motions needed for initiation via RbpA contacts. Elife., 7, e34823. (incl. figure supplements)

Bottoms S, Dickinson Q, McGee M, Hinchman L, Higbee A, Hebert A, Serate J, Xie D, Zhang Y, Coon JJ, Myers CL, Landick R, Piotrowski JS. 2018. Chemical genomic guided engineering of gamma-valerolactone tolerant yeast. Microb Cell Fact., 17, 5.

Ray-Soni A, Mooney RA, Landick R. 2017. Trigger loop dynamics can explain stimulation of intrinsic termination by bacterial RNA polymerase without terminator hairpin contact. Proc. Natl. Acad. Sci. U. S. A., 114, E9233-E9242. (incl. supplement)

Harwig A, Landick R, Berkhout B. 2017. The battle of RNA synthesis: virus versus host. Viruses., 9, E309.

Welch R, Chung D, Grass J, Landick R, Keles S. 2017. Data exploration, quality control and statistical analysis of ChIP-exo/nexus experiments. Nucleic Acids Res., 45, e145. (incl. supplement)

Mishanina TV, Palo MZ, Nayak D, Mooney RA, Landick R. 2017. Trigger loop of RNA polymerase is a positional, not acid-base, catalyst for both transcription and proofreading. Proc. Natl. Acad. Sci. U. S. A., 114, E5103-E5112. (incl. supplement)

Feklistov A, Bae B, Hauver J, Lass-Napiorkowska A, Kalesse M, Glaus F, Altmann KH, Heyduk T, Landick R, Darst SA. 2017. RNA polymerase motions during promoter melting. Science., 356, 863-866. (incl. supplement; Movie S1)

Steinert H, Sochor F, Wacker A, Buck J, Helmling C, Hiller F, Keyhani S, Noeske J, Grimm S, Rudolph MM, Keller H, Mooney RA, Landick R, Suess B, Furtig B, Wohnert J, Schwalbe H. 2017. Pausing guides RNA folding to populate transiently stable RNA structures for riboswitch-based transcription regulation. Elife., 6, e21297. (incl. supplement)

Kohler R, Mooney RA, Mills DJ, Landick R, Cramer P. 2017. Architecture of a transcribing-translating expressome. Science., 356, 194-197. (incl. supplement)

Tetone LE, Friedman LJ, Osborne ML, Ravi H, Kyzer S, Stumper SK, Mooney RA, Landick R, Gelles J. 2017. Dynamics of GreB-RNA polymerase interaction allow a proofreading accessory protein to patrol for transcription complexes needing rescue. Proc. Natl. Acad. Sci. U. S. A., 114, E1081-E1090. (incl. supplement)

Sato TK, Tremaine M, Parreiras LS, Hebert AS, Myers KS, Higbee AJ, Sardi M, McIlwain SJ, Ong IM, Breuer RJ, Narasimhan RA, McGee MA, Dickinson Q, La Reau A, Xie D, Tian M, Piotrowski JS, Reed JL, Zhang Y, Coon JJ, Hittinger CT, Gasch AP, Landick R. 2016. Directed evolution reveals unexpected epistatic interactions that alter metabolic regulation and enable anaerobic xylose use by Saccharomyces cerevisiae. PLoS Genet., 12, e1006372. (incl. supplement and erratum, published in PLoS Genet., 12, e1006447.)

Ghosh IN, Landick R. 2016. OptSSeq: High-throughput sequencing readout of growth enrichment defines optimal gene expression elements for homoethanologenesis. ACS Synth. Biol., 5, 1519-1534. (incl. supplement)

McIlwain SJ, Peris D, Sardi M, Moskvin OV, Zhan F, Myers K, Riley NM, Buzzell A, Parreiras LS, Ong IM, Landick R, Coon JJ, Gasch AP, Sato TK, Hittinger CT. 2016. Genome sequence and analysis of a stress-tolerant, wild-derived strain of Saccharomyces cerevisiae used in biofuels research. G3 (Bethesda)., 6, 1757-1766. (incl. supplement; supplemental tables)

Ray-Soni A, Bellecourt MJ, Landick R. 2016. Mechanisms of bacterial transcription termination: all good things must end. Annu. Rev. Biochem., 85, 319-347.

Zhang J, Landick R. 2016. A two-way street: regulatory interplay between RNA polymerase and nascent RNA structure. Trends Biochem. Sci. 41, 293-310.

Ronayne EA, Wan YC, Boudreau BA, Landick R, Cox MM. 2016. P1 ref endonuclease: a molecular mechanism for phage-enhanced antibiotic lethality. PLoS Genet. 12, e1005797.

Harden TT, Wells CD, Friedman LJ, Landick R, Hochschild A, Kondev J, Gelles J. 2016. Bacterial RNA polymerase can retain sigma70 throughout transcription. Proc. Natl. Acad. Sci. U. S. A. 113, 602-607. (incl. supplement)

Full publications list