The Landick Lab
University of Wisconsin-Madison
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Charles Yanofsky Professor
of Biochemistry & Bacteriology
5441 Microbial Sciences
1550 Linden Dr.
University of Wisconsin
Madison, WI 53706-1567
Ph. 608 265 8475
Fax 608 262 9865
University of Wisconsin-Madison
Department of Biochemistry
Department of Bacteriology
Department of Biomolecular Chemistry
iPIB - Integrated Program in Biochemistry
Microbiology Doctoral Training Program
CMB Training Program
Microbial Genome Biology Focus Group (CMB)
Genetics Training Program
Biophysics Training Program
Molecular Biosciences Training Program
Biotechnology Training Program
Great Lakes Bioenergy Research Center
Micro/Biochem 612 Resources
Landick Lab News
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Welcome to the Landick Lab
Our research focuses on (1) RNA polymerase, the central enzyme of gene expression in all free-living organisms; (2) mechanisms by which gene expression by RNA polymerase is regulated and can be re-programmed for biodesign; and (3) applications of these basic research advances to microbial biotechnology and to antibiotic discovery. Our basic research focus is to understand how the fundamental properties of RNA polymerase, largely conserved from bacteria to human, make it susceptible to pausing, arrest, or termination and how elongation regulators, nucleoprotein structures, and metabolic, developmental, and environmental signals alter these properties. We use a variety of approaches, including genetics, biomolecular chemistry, synthetic biology, systems biology, biophysics, and structural biology, to study both fundamental and applied paradigms of gene regulation. Lab members develop and apply expertise on one or more approach to both individual and collaborative projects. Follow links here to learn more about our research and our lab.
Check out our latest publications
Ray-Soni, A., Bellecourt, M. J., and Landick, R. 2016. Mechanisms of bacterial transcription termination: All good things must end. Annu. Rev. Biochem., 85, 319-347.
Zhang, J., and Landick, R. 2016. A two-way street: Regulatory interplay between RNA polymerase and nascent RNA structure. Trends Biochem. Sci., 41, 293-310.
Harden, T.T., Wells, C.D., Friedman, L.J., Landick, R., Hochschild, A., Kondev, J., and Gelles, J. 2016. Bacterial RNA polymerase can retain sigma70 throughout transcription. Proc. Natl. Acad. Sci. USA, 113, 602-607.
Bae, B., Feklistov, A., Lass-Napiorkowska, A., Landick. R., and Darst, S. A. 2015. Structure of a bacterial RNA polymerase holoenzyme open promoter complex. eLife, 4, e08504.
Bae, B., Nayak, D., Ray, A., Mustaev, A., Landick, R., and Darst, S. A. 2015. CBR antimicrobials inhibit RNA polymerase via at least two bridge-helix cap-mediated effects on nucleotide addition. Proc. Natl. Acad. Sci. USA, 112, e4178-87.
Kotlajich, M.V., Hron, D.R., Boudreau, B. A., Sun, Z., Lyubchenko, Y., and Landick, R.. 2015. Bridged filaments of histone-like nucleoid structuring protein pause RNA polymerase and aid termination in bacteria. eLife, 4, e04970.
Windgassen, T.A., Mooney, R.A., Nayak, D., Palangat, M., Zhang, J., and Landick, R. 2014. Trigger-helix folding pathway and SI3 mediate catalysis and hairpin-stabilized pausing by Escherichia coli RNA polymerase. Nulceic Acids Res. 42, 12707-12721.
Hein, P.P., Kolb, K.E., Windgassen, T., Bellecourt, M.J., Darst, S.A., Mooney, R.A., and Landick, R. 2014. RNA polymerase pausing and nascent-RNA structure formation are linked through clamp-domain movement. Nat. Struct. Mol. Biol. 21, 794-802.
Larson, M.H., Mooney, R.A., Peters, J.M., Windgassen, T., Nayak, D., Gross, C.A., Block, S.M., Greenleaf, W.J., Landick, R., and Weissman, J.S. 2014. A pause sequence enriched at translation start sites drives transcription dynamics in vivo. Science 344, 1042-1047.
Haft, R.J., Keating, D.H., Schwaegler, T., Schwalbach, M.S., Vinokur, J., Tremaine, M., Peters, J..M., Kotlajich, M.V., Pohlmann, E.L., Ong, I.M., Grass, J.A., Kiley, P.J., and Landick, R. 2014. Correcting direct effects of ethanol on translation and transcription machinery confers ethanol tolerance in bacteria.. Proc. Natl. Acad. Sci. USA 111, E2576-2585.
Czyz, A., Mooney, R.A., Iaconi, A., and Landick. R.. 2014. Mycobacterial RNA polymerase requires a U-tract at intrinsic terminators and is aided by NusG at suboptimal terminators. mBio 5, e00931.
Zhang, Y., Mooney, R.A., Grass, J.A., Sivaramakrishnan, P., Herman, C., Landick, R., Wang, J.D. 2014. DksA guards elongating RNA polymerase against ribosome-stalling-induced arrest. Mol. Cell 53, 766-778.
Kolb, K. E., P. P. Hein, and R. Landick. 2014. Antisense oligonucleotide-stimulated transcriptional pausing reveals RNA exit channel specificity of RNA polymerase and mechanistic contributions of NusA and RfaH. J. Biol. Chem. 289, 1151-1163.
Nayak,D., M. Voss, T. Windgassen, R. A. Mooney, and R. Landick. 2013. Cys-pair reporters detect a constrained trigger loop in a paused RNA polymerase. Mol. Cell 50, 882-893.
Srivastava,D. B., K. Leon, J. Osmundson, A. L. Garner, L. A. Weiss, L. F.
Westblade, M. S. Glickman, R. Landick, S. A. Darst, C. L. Stallings,
and E. A. Campbell. 2013. Structure and function of CarD, an essential
mycobacterial transcription factor. Proc. Natl. Acad. Sci. USA 110, 12619-12624.
Chung,D., D. Park, K. Myers, J. Grass, P. Kiley, R. Landick, and S. Keles. 2013. dPeak: high resolution identification of transcription factor binding sites from PET and SET ChIP-seq data. PLoS Comput. Biol. 9, e1003246.
Park,D. M., M. S. Akhtar, A. Z. Ansari, R. Landick, and P. J. Kiley. 2013. The bacterial response regulator ArcA uses a diverse binding site architecture to regulate carbon oxidation globally. PLoS Genetics 9, e1003839.
Myers,K. S., H. Yan, I. M. Ong, D. Chung, K. Liang, F. Tran, S. Keles, R. Landick, and P. J. Kiley. 2013. Genome-scale Analysis of Escherichia coli FNR Reveals Complex Features of Transcription Factor Binding. PLoS Genetics 9, e1003565.